SMTP (linux)
SMTP is a plain text protocol. This makes it easy to simulate a mail client with the telnet command to check the access to port 25.
Test Access to Mail Server with Telnet
$ telnet localhost 25 Trying ::1... Connected to localhost. Escape character is '^]'. 220 corelogs.its.fsu.edu ESMTP Postfix ehlo localhost 250-localhost 250-PIPELINING 250-SIZE 157286400 250-VRFY 250-ETRN 250-STARTTLS 250-ENHANCEDSTATUSCODES 250-8BITMIME 250-DSN 250 SMTPUTF8 mail from: no-reply@localhost 250 2.1.0 Ok rcpt to: dave@davehome.net 250 2.1.5 Ok data 354 End data with <CR><LF>.<CR><LF> Subject: Test Test body. . 250 2.0.0 Ok: queued as 3A83B1102528 quit 221 2.0.0 Bye Connection closed by foreign host.